The first Olympic Games in Greece, in '1896, make the conditions for the creation of an athletic club in the city of Karditsa.

The "Laikos Athlitikos Syllogos i Athina" (Greek: Λαϊκός Αθλητικός Σύλλογος η Αθηνά) (=popular sports association "Αthena") ripe the conditions for the creation of an athletic football club in Karditsa.

[2] From its inception in 1904, the football team of Anagennisi[3] (Athlitikos Syllogos Anagennisis=>ASA=>Sport Assosiation Anagenisi) was not officially recognized as a distinct entity within the organization.

Playing in the independent, regional Thessalian League from 1929 to 1962, it joined the newly established Greek second division in time for the 1962–63 season.

They hold the record for the most fans to ever attend an away game for Gamma Ethniki in 2008, with 2.000 supporters in the play-off match against Ilioupoli.

[11][12] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.