No congregation prays regularly at the Ancient Synagogue, however it is used for festive occasions, such as B'nei Mitzvah and weddings.

[10] Shlomo ben Aderet, leader of Spanish Jewry of his time, served as the rabbi of the Sinagoga Major for 50 years during the late 13th century.

His research was based on a reconstruction of the route followed by a thirteenth-century tax collector that ended at the Sinagoga Major.

Iaffa noted that the structure had been built in compliance with religious requirements that the building should face Jerusalem and that it should have two windows.

[5] In January 2009, a right-wing extremist affiliated with the Republican Social Movement attacked the Sinagoga Major.