It is the story about a little girl who has a doorway to another world in her room, from which three bizarre and frequently-annoying stuffed animal-like creatures emerge to make her life "interesting".

[2] It is explained in the anime that AniYoko itself is an alternative world inhabited by stuffed-animal-like creatures.

The only method of travel between AniYoko and the human world is through a door mounted on the floor of Ami's bedroom.

The Trio has given many conflicting and spurious explanations for how the door got there, but the real answer may never be revealed to Ami nor the viewer.

Ami and the Trio frequently find that they can go to many other places, such as a jungle, a forest, a baseball stadium (complete with crowd) and an RPG-style dungeon, without actually leaving the room.