Margravine Anna of Brandenburg (27 August 1487 – 3 May 1514) was a noblewoman from the Holy Roman Empire.

Margravine Anna was the daughter of John Cicero, Elector of Brandenburg and Margaret of Thuringia.

In 1500 she was betrothed to Frederick, then Duke of Schleswig and Holstein and, after her death, king of Denmark and Norway.

The marriage, held in Stendal, was a double one: on the same day, Anna's brother Joachim and Frederick's niece Elisabeth were married.

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