She was the second wife of Henry Radcliffe, 2nd Earl of Sussex, who divorced her in 1555 on the grounds of her alleged bigamous marriage to Sir Edmund Knyvet, and her "unnatural and unkind" character.
She served as a lady-in-waiting in the household of Queen Consort Katherine Parr, the sixth wife of King Henry VIII, and shared her Reformed beliefs.
[1] An aunt, Agnes Calthorpe, had thirdly been married as his first wife to Charles Knyvett (died 1528), the great-uncle of Anne's alleged bigamous husband Sir Edmund.
He was a second cousin of King Henry VIII of England as they shared Richard Woodville, 1st Earl Rivers and Jacquetta of Luxembourg as great-grandparents.
In a letter written by Anne to her mother, she alleged that Radcliffe had thrown her out of his house without "money, men, women, or meat, and no more than two velvet gowns".
In 1555, he tried again with yet another Bill, this time to prevent her from enjoying her dowry or jointure rights which did pass; however, he no longer sought to bastardise her children.
The following year, a Bill of Parliament settled the matter of her jointure, and by June 1559 she had married Andrew Wyse, a former Royal Officer in Ireland.