Besides acting, he also does a Radio show, named Suhaana Safar With Annu Kapoor which is broadcast on 92.7 Big FM.

[6] His father owned a travelling Parsi theatre company which performed in cities and towns, and his mother was an Urdu teacher and a trained classical singer.

Following a stint there, he joined National School of Drama in 1976, after his brother Ranjit Kapoor, who was already a student there insisted.

[16] Subsequently he starred in many critically and commercially acclaimed films like Mr. India (1987), Tezaab (1988), Ram Lakhan (1990), Ghayal (1990), Hum (1991), Darr (1993), Sardar (1993), Om Jai Jagadish (2002), Aitraaz (2004) and 7 Khoon Maaf (2011).

[17][18] Kapoor began his career as a stage actor in 1979 and was noticed in 1984's Ek Ruka Hua Faisla, directed by Basu Chatterjee and written by elder brother Ranjeet.

His career has spanned over 30 years, including Bollywood cinema, television serials and game shows.

Kapoor is best remembered for hosting the singing show Close-Up Antakshari, produced by Zee TV.

After Vicky Donor, he starred in Yamla Pagla Deewana 2 (2013), Shaukeen (2014), Saat Uchakkey (2016), Jolly LLB 2 (2017), Missing (2018), Dream Girl (2019), Khuda Haafiz (2020) and Suraj Pe Mangal Bhari (2020).

His range of roles varies from portrayal as Saint Kabir in DD National TV serial of the same name, as Mahatma Gandhi in Sardar and Veer Savarkar in Kaala Pani, to police inspector in 7 Khoon Maaf, a petty thief in Utsav to comedic villain in Hum.

The Golden Era – With Annu Kapoor on the Mastiii channel is a nostalgia TV show about Indian cinema, adorned with classical era's Hindi film songs and commentary by Annu Kapoor on unheard tales of Bollywood Cinema's Legends.