She was one of the 13 legitimately born children from his marriage to Beatrice Regina della Scala from the Scaliger family, the lords of Verona.
Antonia's maternal grandparents were Mastino II della Scala and his wife Taddea da Carrara.
He seized the papal city of Bologna, rejected the Pope and his authority, confiscated ecclesiastical property, and forbade any of his subjects to have any dealings with the Curia.
[5] Antonia laid out water gardens in their castle grounds, known as "der Frau von Mailand Garten".
Eberhard remarried after Antonia's death to Elisabeth, daughter of John III, Burgrave of Nuremberg and Margaret of Bohemia.