After leaving school in 1902, he considered entering the seminary, however he was persuaded to delay his entry into the priesthood on the advice of his advisor and confessor Father Corrado, who wanted Barluzzi to further his education first.

While in Jerusalem he was asked by Father Razzoli, head of the Franciscan Custody of the Holy Land, to submit plans for a basilica on Mount Tabor.

After leaving seminary he joined the Italian army as a Sergeant and began his military service with the Fortifications Office overseeing archaeological excavations at the second-century Castel Sant'Angelo.

While in Palestine he met Father Ferdinando Diotallevi, the new Custos, or head of the Custody of the Holy Land, who had the plans Barluzzi had previously drawn for Mount Tabor during his first visit.

Barluzzi eventually received the commission for the Nazareth project, and when this decision was revoked in 1958, "he suffered a heart attack which brought on cerebral deafness and pulmonary emphysema".

His funeral was celebrated at the Basilica di Sant'Antonio at Lateran and was attended by Cardinal Gustavo Testa, the procurator General of the Franciscan Order and former Apostolic Delegate in Palestine.