Antrolana lira, also known as The Madison Cave isopod, is a freshwater, cave-dwelling crustacean species.

[5] It is inferred that the species has a low reproductive potential, as the majority of samples consist of very few to no juveniles and no egg-bearing females.

[5][3] Based on sampling data, populations of Madison Cave isopods tend to skew towards a female-biased sex ratio, ranging from 2.2 to 3.7 females per male.

However, it has been suggested that this sex ratio may be due to differential responses of females and males to shrimp bait.

[5] The feeding habits of Madison Cave isopods are unknown, but they are believed to be carnivorous as they readily consume shrimp bait.

Plates of calcite are often found floating on the surface of the aquifers it occupies, which indicates that the water is supersaturated with calcium carbonates.

Heavy folding and faulting in the Shenandoah Valley area suggests limited connectivity between the aquifers in which the Madison Cave isopod is found.

Urban development in the northern part of Madison Cave isopod's range and agricultural runoff in the Shenandoah Valley increases the probability of pollutants entering the groundwater.

Fourteen new homes and a large church were built in the recharge area of the Front Royal Caverns aquifer.

The exact recharge zones of the aquifers at any location with Madison Cave isopods are unknown, so the total impact of pollution into groundwater is difficult to predict.

A report by Bolgiano found that the isopod were threatened by human vandalism and mercury pollution in the South River.