The Apostolic Vicariate of Gambella (Latin: Vicariatus Apostolicus Gambellensis) (formerly the Apostolic Prefecture of Gambella) is a Catholic missionary pre-diocesan jurisdiction (known as an apostolic vicariate) in the western part of Ethiopia.

It is exempt, i.e. immediately dependent upon the Holy See (notably the Roman Congregation for the Evangelization of Peoples), not part of any ecclesiastical province (such as the Metropolitan Archbishopric of Addis Abbeba).

The Apostolic Vicariate of Gambella comprises all the Gambella Region and large part of Illubabor Zone in Oromia Region.

On 5 December 2009 it was promoted an apostolic vicariate,[1] since entitled to a titular bishop.

(Roman Rite, so far missionary members of a Latin congregation)