In Egypt, the type locality of this spider, females were found surviving the relatively warm winter months.

In Illinois, Argiope trifasciata hatches in early summer but does not become readily notable until mid-August, when they have grown large enough to make their distinctive webs, which can be up to 60 cm (24 in) in diameter, among stems and bushes.

The female rests at the centre of the web facing downwards, with her legs often arranged in pairs, making a cross shape; some female spiders conceal themselves in a hidden location close to the web, being alerted to a potential victim by a non-sticky thread leading to the center.

Large, powerful prey like paper wasps, are swiftly wrapped in silk to immobilise them, before being injected with toxic saliva.

[8] Even though the purpose behind the silk decorations made by Argiope trifasciata remains uncertain, there are a few hypotheses: to make the spider appear larger and to act as a warning sign.