He is also well known for the development of various biochemical research techniques, most notably the differential display methodology, which is used in examining the activation of genes in cells.
More recently he championed the acceptance and adoption of the conceptual review as a valuable approach to unearthing new knowledge from the enormous stores of information in the scientific literature.
Prior hypotheses around the translation of genetic information into proteins had focused on ribosomes, which turned over too slowly to enable the rapid synthesis seen in PaJaMo.
On 15 April 1960, Jacob discussed the PaJaMo experiment's findings with Sydney Brenner and Francis Crick at King's College, Cambridge.
[9] With his student Richard Yates, Pardee discovered that biosynthesis of pyrimidine in Escherichia coli is subject to feedback inhibition.