Ashiura Kannon-ji (芦浦観音寺) is a Buddhist temple of the Tendai sect located in the city of Kusatsu, Shiga Prefecture, Japan.

According to the temple's legend, it was founded by Prince Shōtoku and Hata no Kawakatsu and belonged to the Sanron sect of early Japanese Buddhism.

The location of the temple is strategic point for amphibious and land transportation in the southeastern part of Lake Biwa, as it is on the ancient Tōsandō (Nakasendō) and Tōkaidō highways connecting the capital of Heian-kyō with the provinces of eastern Japan, as well as the main route from Kyoto to the Ise Grand Shrine.

Toyotomi Hideyoshi named the head abbot of the temple as a daikan and bugyō with control over all shipping on Lake Biwa, and estates in southern Ōmi with a kokudaka of 24,000 koku.

[3] The temple's Shoin is a surviving structure of the early Edo Period Nakahara Goten, and is also a National Important Cultural Property.