The women's 100 metres was the shortest of the four women's track races in the Athletics at the 1964 Summer Olympics program in Tokyo.

45 athletes from 27 nations entered, with 1 not starting in the first round.

The top four runners in each of the four heats advanced to the semifinals.

† In the Second Round results as per the Official Olympic report,[2] Avis McIntosh, of New Zealand, was erroneously mentioned as a Dutch athlete.

The top four runners in each of the two semifinals advanced to the final.