She became the first person to defend the championship at 100 metres, a feat later duplicated by Carl Lewis, Gail Devers, Shelly-Ann Fraser-Pryce, Usain Bolt and Elaine Thompson-Herah.
Director Bud Greenspan filmed Tyus casually dancing behind her starting blocks before the Olympic final.
In the final, American teenager Margaret Bailes gained a step advantage at the gun.
The next chasers appeared to be her American teammate Barbara Ferrell and Australian teenager Raelene Boyle.
Ferrell and Boyle were escorted to the holding area, but Officials reading the new fully automatic time system corrected the results to declare Kirszenstein the bronze medalist.