The women's 400 metres event at the 1995 Summer Universiade was held on 31 August – 1 September at the Hakatanomori Athletic Stadium in Fukuoka, Japan.
[1] Note: Du Xiujie of China originally won the silver with 50.72 but was later disqualified for doping.
[2] Qualification: First 3 of each heat (Q) and the next 1 fastest (q) qualified for the semifinals.
Qualification: First 4 of each semifinal qualified directly (Q) for the final.