Having swept the 400 metres and winning the World Championships as part of their qualifying process, defending champion USA came in as an overwhelming favorite.
USA put their Olympic champion LaShawn Merritt on the leadoff leg with the intent to break the race wide open from the start.
A 45 second runner, Belgium's Cédric Van Branteghem was no match as Neville pulled away while Bahamas Andrae Williams closed in from behind.
Brown was in the sights of Russia's Denis Alekseyev through the final turn until he opened up a clear second place down the home stretch.
Taking the baton in 6th place, Great Britain's 400 metre finalist Martyn Rooney worked his way past Jamaica and Belgium, almost catching Alekseyev at the line.