The Men's 400 metres T44 event at the 2012 Summer Paralympics took place at the London Olympic Stadium from 7 to 8 September.

[1] The T44 category is for athletes who have a single below knee amputation or who can walk with moderately reduced function in one or both legs.

Prior to the competition, the existing world and Paralympic records were as follows: Competed 7 September 2012 from 21:45.

rule: first 3 in each heat (Q) plus the 2 fastest other times (q) qualified.

PR = Paralympic Record.