The mixed 4 × 100 metres relay event at the 2020 Summer Paralympics in Tokyo, took place on 3 September 2021.

Prior to the competition, the existing records were as follows:[1] Heat 1 took place on 3 September 2021, at 12:10:[2] Heat 2 took place on 3 September 2021, at 12:23:[3] Heat 3 took place on 3 September 2021, at 12:36:[4] The final took place on 3 September 2021, at 21:17:[5]