The women's 100 metres T35 event at the 2020 Summer Paralympics in Tokyo, took place on 27 August 2021.

The T35 category is for ambulant athletes with cerebral palsy.

The typical T35 athlete may need assistive devices for walking.

The athlete may have sufficient function to run but demonstrates poor balance.

Prior to the competition, the existing records were as follows:[1] Heat 1 took place on 27 August 2021, at 10:00:[2] Heat 2 took place on 27 August 2021, at 10:07:[3] The final took place on 27 August 2021, at 12:45[4]