The women's 800 metres T54 event at the 2020 Summer Paralympics in Tokyo, took place on 29 August 2021.

The T54 category is for wheelchair athletes with no leg function, but near full arm function and reasonable to normal trunk function.

Typically this may be caused by a lower spinal cord injury or spinal cord birth defect.

Prior to the competition, the existing records were as follows:[1] Heat 1 took place on 29 August 2021, at 10:57:[2] Heat 2 took place on 29 August 2021, at 11:07:[3] The final took place on 29 August 2021, at 19:17:[4]