Rio Negro is the second oldest club of Amazonas state, and is eleven months younger than its rival, Nacional.

[1] Atlético Rio Negro Clube of Roraima state is named after the club, and shares the same logo, colors and kits.

[2] On November 13, 1913, 14-year-old Shinda Uchôa and his friends founded Atlético Rio Negro Clube at Manuel Afonso do Nascimento (who was one of the founders) home.

[5] In the following year, in 1974, Rio Negro finished in Campeonato Brasileiro Série A's 26th position, ahead of Botafogo.

[3] There are several ultra groups supporting the club:[12] Besides football, Rio Negro also has other sports sections, such as basketball, volleyball, gymnastics, and futsal.