After an initial run from 2005 to 2013 (which originally aired from 2005 until 2013, weeknights on G4, G4 Canada, Fuel TV, HOW TO Channel, and Maxxx), the show was revived in 2021 and canceled again when the network closed in 2022.

[1] The second incarnation of the program featured an ensemble cast, including returning original host Kevin Pereira, Kassem G, Fiona Nova, Gina Darling, Will Neff, and Austin Creed.

After a brief montage of clips from AOTS history and final goodbyes from the show's hosts and staff, the series ended with former Screen Savers hosts Patrick Norton and Leo Laporte in a coffee shop, acting as if the entire run of AOTS had been a dream.

They then don "rocket packs" and fly off to work in a skyscraper adorned with the old TechTV logo, as the theme song of The Screen Savers plays.

Afterwards, text came up on the screen against a gray background that read, "To our fans, our haters, and the Internet, thanks for seven kick-ass years.

On December 29, 2019, NBC's San Francisco-owned station KNTV aired a 30-minute special titled Best of Attack of the Show at 2:00am.

[10] A long-form-interview-only version called Attack of the Show: The Loop premiered on November 17, 2021, featuring Adam Savage as guest.

Another version called Attack of the Show: Vibe Check premiered on November 23, 2021, which featured a rotating selection of hosts sitting at a table discussing various topics and answering viewer questions; this edition was streamed live on Tuesdays.

Zach Selwyn, the runner up on ESPN's Dream Job, was hired to replace Moran and started his hosting duties a week later on April 17, 2006.

After the departure of Munn, AOTS featured several guest co-hosts including: Candace Bailey, Jessica Chobot, Alison Haislip, Carrie Keagan, Alessandra Torresani, Sara Underwood, Milynn Sarley, and Morgan Webb.

On May 9, 2012, Pereira announced on-air that he was officially leaving AOTS to pursue work in television production.

He stated, "I plan to turn my attention to full-time with my production company Super Creative and live touring nerd-carnival leetUP."

[15] Beginning August 28, 2012, the show debuted a final new opening credit, with no reference to permanent or guest hosts.

[17][18] They are regularly joined by Austin Creed, with other network hosts like Ovilee May, Alex "Goldenboy" Mendez, Jirard "The Completionist" Khalil, and AustinShow occasionally making appearances.