The company operated a regular passenger and airmail service between Brisbane, Sydney and Melbourne that was in January 1931 extended to Launceston and Hobart in Tasmania.

[3] Unable to obtain a formal mail subsidy, the deepening Great Depression saw revenues fall, a situation that worsened after the crash of VH-UMF Southern Cloud in the Australian Alps between Sydney and Melbourne on 21 March 1931.

ANA ceased scheduled services at the end of June 1931,[4] although it continued to operate joy flights mostly around New South Wales,[5] and offered pilot training services with a fleet of small aircraft.

[6] Late in 1931 ANA attempted to open an Australia-England airmail service with a special Christmas airmail flight that was interrupted by the crash of VH-UNA Southern Sun in Malaya.

After lengthy efforts to interest the Australian Government in subsidising a regular Australia-UK airmail service failed, ANA went into voluntary liquidation in April 1933, and its remaining assets were sold off.