The crew consisted of Michael López-Alegría,[6] a Spaniard-American and a professionally trained astronaut hired by Axiom, Eytan Stibbe[7] from Israel,[8] Larry Connor from the United States,[8] and Mark Pathy from Canada.

[16] On 26 January 2021, Axiom revealed the full crew of the mission, consisting of Michael López-Alegría, Larry Connor, Mark Pathy and Eytan Stibbe.

[25] It was reported that actor Tom Cruise and film producer Doug Liman would be passengers for a movie project,[26] but it was later announced that they would fly on a subsequent flight.

[2] It was revealed that the "zero-g indicator" aboard the first private mission to visit the International Space Station was a toy dog called Caramel, the mascot for the Montreal Children's Hospital Foundation.

[32] The Israeli mission segment is called Rakia, which means "sky" in Hebrew and is also the title of the book published with Ilan Ramon's diary fragments that survived the 2003 Space Shuttle Columbia disaster.

With Connor being an Ohio-native, the items included a John Glenn senatorial campaign button, a patch of the Armstrong Air & Space Museum, and a piece of Kapton foil removed from the Apollo 11 Command Module after splashdown.