In addition to holding senior management roles in the private sectors and managing the Nigerian Women Trust Fund – a non-profit organization focused on increasing the quality and quantity of women in decision-making, for three years, Osori has consulted for the World Bank, United Nations Development Programme, Department for International Development, UNICEF and the National Democratic Institute.

[citation needed] In 2015 she was chosen to be one of 21 women who met for a conference at Harvard University Kennedy School of Government funded by Hunt Alternatives.

The group included Fauzia Nasreen from Pakistan, Judy Thongori from Kenya and Olufunke Baruwa, Esther Ibanga and Hafsat Abiola also from Nigeria.

[citation needed] In 2018, the Open Society Foundations announced the appointment of Osori as the executive director of the Open Society Initiative for West Africa (OSIWA) overseeing the operations of OSIWA in 10 African countries; Benin, Ivory Coast, Ghana, Guinea, Liberia, Mali, Niger, Nigeria, Sierra Leone, and Senegal.

[10] In 2018, Ayisha Osori co-authored a book titled Too Good to Die: Third Term And The Myth Of The Indispensable Man In Africa, with the former chairman of the National Human Rights president Chidi Odinkalu.