The Aylesbury Vale (or Vale of Aylesbury) is a geographical region in Buckinghamshire, England, which is bounded by the City of Milton Keynes and West Northamptonshire to the north, Central Bedfordshire and the Borough of Dacorum (Hertfordshire) to the east, the Chiltern Hills to the south and South Oxfordshire to the west.

The Silverstone Circuit sits on the northern boundary of the Vale with South Northamptonshire, and the Buckinghamshire Railway Centre is located near Quainton.

The A421 road passes through the north of the Vale providing connections to Milton Keynes, Bedford and the M1 to the east, and the M40, Oxford and Birmingham to the west.

In addition, the Chiltern Main Line passes through the district, stopping at Haddenham & Thame Parkway.

EWR runs across the Vale, with trains between Oxford and Milton Keynes Central, calling at Winslow and Bletchley, due to commence in early 2025.

(The route of HS2 uses the same corridor and, as of August 2023[update], the Claydon line is needed to move materials for its engineering works.)