The GWR provided motive power and trains to both the Wycombe Railway and the A&B; it ran a shuttle service from Princes Risborough to Verney Junction.

The original junction layout on the route to London Marylebone included a sharp curve because the station had been a terminus for the Metropolitan Railway.

Rather than change the junction layout to suit faster trains, a 15 mph (24 km/h) speed restriction was applied to the curve.

There was heavy fog at the time of the accident and, at the subsequent Board of Trade inquiry, there was some doubt as to how well driver Barnshaw knew the route.

[3] What the inquiry did not touch on was that there had been a history of fast running of these newspaper trains, which had become an important traffic for the Great Central Railway.

The Wycombe Railway opened a single-road engine shed, which was fifty feet in length, to the west of the station in 1863.

This was extended to the rear shortly afterwards and enlarged to a two-road shed by the Great Western Railway in 1871; in around 1893, the saw tooth roof was added on the original walls.

From 1948 to 1961, Aylesbury was the terminus of the Met's main line, on which trains had to change between electric and steam locomotives at Rickmansworth.

In 1966, British Railways closed the Great Central Main Line north of Aylesbury, leaving the town with commuter services to London only.

A new driver's staff room was established on platform 3 and a new heavy maintenance depot was built just north of the station.

On selected days, usually bank holidays, special passenger services run to the Buckinghamshire Railway Centre at Quainton Road.

From 21 January 2008, the taxi rank was moved to the car park for 52 weeks as a result of major engineering work on the new Southcourt Bridge and the new Station Boulevard.

[citation needed] These units are used jointly by Chiltern Railways and Network Rail for route learning and Sandite duties.

In 2004, a 'regional planning guidance' report, written by consultants for Buckinghamshire County Council concerning the development of Aylesbury Vale, recommended further expansion of rail services to Bletchley and Bedford.

The majority of bus routes are operated by Arriva Herts & Essex, which Connect the town with several destinations across Buckinghamshire, Hertfordshire and Oxfordshire, including Stoke Mandeville Hospital, Milton Keynes, Oxford, Tring, Hemel Hempstead, Watford, Luton and Leighton Buzzard.

This route was greatly improved by Project Evergreen, the redualing and speeding-up of Marylebone – Risborough – Birmingham track and services.