When the first section of the Oxford and Rugby Railway was opened as far as Banbury on 2 September 1850,[1][2] there were only three intermediate stations, the northernmost of which was Aynho.

[5] To the north of the station is Aynho Junction, the northern end of the Bicester "cut-off" line, which was brought into use in 1910.

[6][8] Another fatality occurred at the station in August 1942 when Baroness Lilian Helen van Lennep (a Dutch national), who had been staying with Lady Fairfax Cartwright (widow of Fairfax Leighton Cartwright) at The Grammar House, was struck by an express train while crossing the line via the barrow crossing.

[9] The station passed on to the Western Region of British Railways on nationalisation in 1948.

It was then closed by the British Railways Board, to goods on 4 May 1964 and to passengers on 2 November 1964, along with three other stations between Leamington Spa and Didcot; by this time it had been renamed Aynho for Deddington.