The left wall shows the Annunciation, set in a large Renaissance loggia whose pavement, depicted in geometrical perspective, leads, behind the hortus conclusus, to a richly detailed landscape.
The main characters are conventional ones: Mary is distracted from reading by an angel, who kneels to bless her with a white lily in one hand, a symbol of her virginal purity.
In the upper part God the Father is depicted, surrounded by angels, emitting a ray of light incorporating the Holy Ghost in the form of a dove.
The right part of the fresco shows a window with a grid; on the left is a shelf with books and an amphora: under it is a self-portrait of Pinturicchio, featuring the dedicatory bejewelled inscription, "BERNARDINVS PICTORICIVS PERVSIN[VS]".
The group of the Madonna with Child re-uses the typology of the Adoration of the Shepherds painted by Pinturicchio in the Presepio Chapel of Santa Maria del Popolo in Rome.
The crowd is formed by standard characters including young spouses, wise men, toothless women and other normal spectators.