Located at 442 Adelaide Street North, it is known as the “Birthplace of Insulin.” It is the house where Sir Frederick Banting woke up at two o'clock in the morning on October 31, 1920 with the idea that led to the discovery of insulin.

He attempted a private medical practice and when it was unsuccessful, he began working at the University of Western Ontario; his research for a lecture there was what inspired his 25 word idea that provided the key to discovering insulin, and prevented the certain death of those affected by diabetes.

Some notable artifacts include Banting's original art, desk, medicine cabinet, and bed frame, as well as his Military Cross, the KBE, and his official replica of the Nobel Prize.

[6][4] For decades, the public viewed the house as the "Birthplace of Insulin," and many individuals expressed their desire to have it turned into a shrine or monument to honour the Canadian hero.

[5] Adjacent to the museum is Sir Frederick G. Banting Square, a garden with several information plaques, and three main attractions.

It represents Canada's gift to the world, and embraces Banting House's visitors from abroad, with the names of every country listed on it.