Strýcová won two WTA singles titles, at the 2011 Tournoi de Québec and the 2017 Linz Open, finishing runner-up on six further occasions.

Strýcová represented the Czech Republic in the Fed Cup from 2002 to 2018, and was a key part of the team which dominated the competition for almost a decade, winning six titles between 2011 and 2018.

[3] Strýcová was a strong junior player, winning two majors in girls' singles, the 2002 Australian Open, and then defending that title the following year.

Turning professional in 2003, Strýcová had already worked her ranking into the top 300 with some good results in ITF Women's Circuit events over 2002.

She continued to play mostly ITF events throughout the year, and made her Grand Slam debut at Wimbledon, qualifying and losing in the first round to Tatiana Perebiynis.

She began the year by qualifying for the Australian Open and then reached the fourth round at the WTA tournament in Indian Wells, beating seeded player Eleni Daniilidou before losing to Justine Henin, a result that broke her into the top 100 for the first time.

After hitting a rough patch in the middle part of the season, she finished the year strongly by reaching her first WTA Tour semifinal in Guangzhou and winning an ITF event in Saint-Raphaël, France.

2006 also begun poorly for her in singles, as she struggled to string together wins and subsequently dropped out of the top 200 of the world rankings in April 2006 before recovering slightly after some good results in ITF events.

In 2007, Záhlavová-Strýcová played mostly on the ITF Circuit once more and achieved some good results, reaching several semifinals throughout the season, but still sat outside the top 100.

She qualified for Wimbledon and made the third round of a Grand Slam for the first time in her career thus returning to the top 100, where she remained until the end of the season, her second top-100 year-end finish.

Záhlavová-Strýcová's results improved during the latter part of the year, starting with a quarterfinal appearance at the WTA Tour event in Bad Gastein, as well as reaching the doubles final in Prague.

She then played a $25k in Trnava, Slovakia, where she reached the semifinals, before qualifying and winning her first-round match at the US Open, losing to Victoria Azarenka in the second round.

Her Wimbledon performance boosted her confidence and thus at the Prague Open she managed to make the first singles final of her career, dismantling Patty Schnyder with the loss of only two games in the semifinals.

In singles competition, she struggled to build on her strong summer results, failing to advance in six of the ten tournaments she played following Prague, among them a first round loss at the US Open to Maria Kirilenko, thus dropping to world No.

In February, Záhlavová-Strýcová was banned for six months, backdated to 16 October 2012 until 15 April 2013, after testing positive for the stimulant sibutramine, which Strýcová alleged got into her system accidentally through her ingestion of the supplement ACAI Berry Thin.

At the Wimbledon Championships, she defeated reigning Australian Open champion Li Na in the third round, in soon-to-be last professional match for world No.

Advancing to a Grand Slam singles quarterfinal for the first time, she lost in straight sets to eventual champion, compatriot Petra Kvitová.

Záhlavová-Strýcová started the year strongly, with a semifinal finish at the Auckland Open and a quarterfinal showing at the Sydney International, losing to Caroline Wozniacki and Tsvetana Pironkova, respectively.

After a couple of early losses at Auckland and Brisbane, Strýcová got as far as the last 16 of the Australian Open, where she was beaten by Victoria Azarenka, and in February to the final at Dubai, where she came runner-up to Sara Errani.

In her next tournament, the Qatar Open, she defeated Kristina Mladenovic in the first round, and then lost to compatriot Petra Kvitová in straight sets.

At the Indian Wells Open, Strýcová won her first round match against Aliaksandra Sasnovich in straight sets and came back from 5–7, 1–3 down to defeat 22nd seed Andrea Petkovic, 5–7, 6–4, 7–5.

Having not won a main draw match in a decade at the French Open, she reached the third round with wins over compatriot Lucie Hradecká and Polona Hercog.

On her way to the final, she got wins against compatriot, 8th seed Karolína Plíšková, Heather Watson, Tsvetana Pironkova, and CoCo Vandeweghe.

At Cincinnati Open, she came away with the women's doubles title with her new partner Sania Mirza while defeating Martina Hingis and CoCo Vandeweghe in the finals.

In January, Strýcová and her doubles partner Markéta Vondroušová reached the semifinals of the Australian Open, but were defeated by Sam Stosur and Zhang Shuai.

On 4 May 2021, Strýcová announced her retirement from tennis whilst leaving open the possibility of a final match at the 2022 Wimbledon Championships, or at a tournament in the Czech Republic.

[15] In April, she entered the 2023 Mutua Madrid Open singles and doubles events, using a protected ranking with previous partner Hsieh Su-wei.

[16] In July, Strýcová and Hsieh won their second Wimbledon doubles title, beating Storm Hunter and Elise Mertens in the final.