These mucoid alkaline secreting glands are arranged as lobules consisting of alveoli lined by cuboidal or columnar epithelium.

[5] These glands lie on the perineal membrane and beneath the bulbospongiosus muscle at the tail end of the vestibular bulb deep to the posterior labia majora.

The intimate relation between the enormously vascular tissue of the vestibular bulb and the Bartholin's glands is responsible for the risk of hemorrhage associated with the removal of this latter structure.

[6] The openings of the Bartholin's glands are located on the posterior margin of the introitus bilaterally in a groove between the hymen and the labium minus at the 4:00 and 8:00 o'clock positions.

[8][9] Earlier he jointly discovered the glands in cows with Joseph Guichard Duverney (1648-1730), a French anatomist.

[9][12][13] The fluid may slightly moisten the labial opening of the vagina, serving to make contact with this sensitive area more comfortable.