It is the 30th film of the DC Universe Animated Original Movies, and is directed by Sam Liu and written by Jim Krieg and Bruce Timm.

[4] Batman (Bruce Wayne) and Nightwing (Dick Grayson) discover that Poison Ivy (Pamela Isley) and Floronic Man (Jason Woodrue) have teamed up and stolen information from S.T.A.R.

Much to their reluctance, they decide to look for Harley Quinn (Harleen Quinzel; Poison Ivy's best friend and Joker's usual sidekick and girlfriend) to find out where the duo is hiding.

HQ, Batman asks Sarge Steel about the kidnapping of a scientist named Dr. Harold Goldblum who has a background in chemistry, botany, and biological warfare.

The criminal duo complain about humanity's negative environmental impact and Floronic Man says once their virus is activated every person on the planet "will have a vested interest in going green".

Contacting A.R.G.U.S., Batman, Nightwing, and Harley head after them to convince Ivy to stop her mad plans since they have the potential to kill all life on Earth.

"[6] The score was composed by Michael McCuistion, Kristopher Carter and Lolita Ritmanis, which was released on CD by WaterTower Records as an exclusive with FYE.

On July 31, a five-issue prequel miniseries called Harley Quinn and Batman was released on a bi-weekly basis via digital download.

Due to the financial success of The Killing Joke, the film was released in theaters for one night only through Fathom Events in the United States on August 14, 2017.

[15] The film received praise for some of the voice cast and throwbacks to past Batman media, but was criticized for its script, tone, and raunchy humor.

[20] Tyler Smith of Battleship Pretension criticized the more explicit sexual elements, writing, "As Batman and Harley Quinn attempts to incorporate more explicit sexuality – what could be considered 'adult content' – into the story, the film takes on the tone of a couple of 13-year-olds constantly high-fiving each other as they take turns writing risqué Harley Quinn fan fiction.

I'm not exactly sure what the filmmakers were trying to achieve by treating the audience to gratuitous upskirt shots of Harley, but it certainly wasn't an attempt to make her a more well-rounded character.