Be'er Milka (Hebrew: בְּאֵר מִלְכָּה) is a moshav in southern Israel.

Located in the central Negev desert close to the Egyptian border, around three kilometres from Kmehin, it falls under the jurisdiction of Ramat HaNegev Regional Council.

The moshav is located in a nature reserve at the location of an ancient well at the confluence of Nahal Lavan and Nahal Nitzana.

[2] In 2001 a gar'in group from Bnei HaMoshavim (associated with HaNoar HaOved VeHaLomed and the Moshavim Movement) began to organize in preparation for founding a new moshav.

[3] The moshav is named after biblical Milka, the sister-in-law of Abraham (Genesis 22:20-23) and grandmother of Rebekah, the wife of Isaac (Genesis 24:47).