Having made her screen debut in the 2009 comedy film American Pie Presents: The Book of Love, she later ventured into television roles.

After 2 Broke Girls, Behrs returned to feature films with the 2015 productions Chasing Eagle Rock and Hello, My Name Is Doris.

She voiced Carrie Williams in the animated film Monsters University (2013) and Moochie in the television series Home: Adventures with Tip & Oh.

[11] Behrs moved to Los Angeles, California, in 2004, to study acting at the UCLA School of Theater, Film and Television.

[11] In 2005, she played Sandy Dumbrowski in a production of Grease at San Francisco's Ray of Light Theatre, and was named Miss Marin County in 2006.

[13] Behrs stars as Caroline Channing, an heiress from Manhattan's Upper East Side, who is forced to become a waitress at a diner in Williamsburg, Brooklyn after her father is arrested for financial wrongdoing.

[16] Her character is a graduate of the Wharton School who teams up with a waitress named Max (Kat Dennings) in a business venture.

[22] Behrs and Kat Dennings hosted the 40th People's Choice Awards, where she also sang Miley Cyrus' "Wrecking Ball".

Behrs made her Manhattan stage debut in June 2016 at the Lucille Lortel Theatre in Halley Feiffer's comedic play, A Funny Thing Happened on the Way to the Gynecologic Oncology Unit at Memorial Sloan-Kettering Cancer Center of New York City.

[26] Behrs released the book The Total Me-Tox: How to Ditch Your Diet, Move Your Body & Love Your Life in May 2017, in which she shares her personal journey towards health and happiness.

In 2020, she began hosting Harmonics with Beth Behrs, a podcast where she interviews country and bluegrass singers and songwriters with guests including Glennon Doyle, Geeta Novotny, Mickey Guyton, Brandi Carlile, Tichina Arnold, Allison Russell and Mary Gauthier.