In 1927, Lazio was the only major Roman club which resisted the Fascist regime's attempts to merge all the city's teams into what would become Roma the same year.

The club played in the first organised Serie A in 1929 and, led by legendary Italian striker Silvio Piola,[8] achieved a second-place finish in 1937 – its highest pre-war result.

[9] Back to Serie A in 1972–73, Lazio immediately emerged as surprise challengers for the Scudetto to Milan and Juventus in 1972–73, only losing out on the final day of the season, with a team comprising captain Giuseppe Wilson, as well as midfielders Luciano Re Cecconi and Mario Frustalupi, striker Giorgio Chinaglia, and head coach Tommaso Maestrelli.

In 1986, Lazio was hit with a nine-point deduction (a true back in the day of the two-point win) for a betting scandal involving player Claudio Vinazzani.

[15][16] The arrival of Sergio Cragnotti in 1992 changed the club's history, due to his long-term investments in new players to make the team a Scudetto competitor.

[19] In addition, Lazio won the Supercoppa Italiana twice and defeated Manchester United in 1999 to win the UEFA Super Cup.

[23] In the 2006–07 season, despite a later-reduced points deduction, Lazio achieved a third-place finish, thus gaining qualification to the UEFA Champions League third qualifying round, where they defeated Dinamo București to reach the group phase, and ended fourth place in the group composed of Real Madrid, Werder Bremen and Olympiacos.

[24] Lazio started the 2009–10 season playing the Supercoppa Italiana against Inter in Beijing and winning the match 2–1, with goals from Matuzalém and Tommaso Rocchi.

[25] Lazio won the 2012–13 Coppa Italia 1–0 over rivals Roma, with the lone goal coming from Senad Lulić in the 71st minute.

[28] In the 2024-25 UEFA Europa League Group Stage, Lazio topped the 36-club table with a record of 6W-1D-1L (19 points), making €20.469 million.

[30] Originally, Lazio wore a shirt which was divided into white and sky blue quarters, with black shorts and socks.

[1] Also on the Foro Italico lies the Stadio dei Marmi, or "marble stadium", which was built in 1932 and designed by Enrico Del Debbio.

In June 2018, Lazio President Claudio Lotito stated that the Biancocelesti "should be granted the same favour and treatment as Roma – the ability to also build a new stadium.

"[36] In June 2019, Lotito was set to present the designs of a potential future stadium for Lazio, named the Stadio delle Aquile.

[38] In October 2022, Lotito provided an update on his Stadio Flaminio project, stating that he is "very attached" to the hope that Lazio will play there again, as having it as their home is "something that evokes our history and our roots.

"[39] In November 2022, Lotito discussed his plans for the Stadio Flaminio at the ‘Sing a Song’ charity event at the Auditorium della Conciliazione in Rome.

[41] In December 2024, Lotito and his collaborators held a meeting with Mayor Roberto Gualtieri and Councilor Alessandro Onorato at the Municipality of Rome in Campidoglio to present their project for the new Stadio Flaminio.

[42] He envisioned developing a state-of-the-art stadium with a capacity of 40,000 to 50,000 spectators and a retractable roof; he also outlined plans for the comprehensive redevelopment of the surrounding Flaminio district.

[43] However, shortly after, "Roma Nuoto" received approval from the Conference of Services after threatening legal action following the long and unjustified delay in the process for their plans for the abandoned stadium; they envision a multi-sport facility for the future of the Stadio Flaminio, including an Olympic-sized swimming pool, padel courts, and a hockey rink, thus competing with Lazio.

[44] In March 2025, Lotito officially deposited his proposal for the currently-defunct Stadio Flaminio to the Municipality of Rome to become Lazio's new home.

[51][52] A minority of Lazio's ultras used to use swastikas and fascist symbols on their banners, and they have displayed racist behaviour in several occasions during the derbies.

Most notably, at a derby of the season 1998–99, laziali unfurled a 50-metre banner around the Curva Nord that read, "Auschwitz is your town, the ovens are your houses".

[65] Piola, who played also with Pro Vercelli, Torino, Juventus and Novara, is also the highest goalscorer in Serie A history, with 274 goals.

[70] Claudio Lotito, the current chairman of Lazio, purchased the club from Cragnotti in 2004, but owned just 26.969% of shares as the largest shareholders at that time.

Unlike Internazionale, Roma and Milan, who were sanctioned by UEFA due to breaches of Financial Fair Play, Lazio passed the regulations held by the administrative body with the high achievements.

Lotito also received a prize that joint awarded by Associazione Italiana Allenatori Calcio and DGS Sport&Cultura, due to Lazio's financial health.

[79] Mizuno would become the team's new sportswear and technical gear provider, with the Biancocelesti receiving €20 million over the next five years as a result of their new agreement with the Japanese company.

[81] In March 2025, Italian Newspaper Il Messaggero reported that Lazio could soon have their own docu-series on Netflix dedicated to the history of the club; from the foundation in 1900 all the way to the modern era.

Several legendary figures would appear in the series, including Giuseppe Signori, Miroslav Klose, and Paolo Di Canio.