The first set of Perl codes of BioPerl was created by Tim Hubbard and Jong Bhak[citation needed] at MRC Centre Cambridge, where the first genome sequencing was carried out by Fred Sanger.

MRC Centre was one of the hubs and birthplaces of modern bioinformatics as it had a large quantity of DNA sequences and 3D protein structures.

In 1995, Brenner organized a BioPerl session at the Intelligent Systems for Molecular Biology conference, held in Cambridge.

BioPerl had some users in coming months including Georg Fuellen who organized a training course in Germany.

Fuellen's colleagues and students greatly extended BioPerl; this was further expanded by others, including Steve Chervitz who was actively developing Perl codes for his yeast genome database.