It was launched on September 7, 1967, by a Delta G rocket from Cape Canaveral Air Force Station in Florida.

[5] Biosatellite 2 carried 13 biological experiments involving insects, frog eggs, plants and microorganisms.

The mission was ended early because of a tropical storm threat in the recovery area and communication problems between the capsule and ground stations.

The main objective of the mission was to determine if the radiation sensitivity of living organisms in space is greater or less than on land, for which disposed of a radiation source in front of the capsule.

The satellite is currently the property of the National Air and Space Museum, reference number A19731629000.