[8] The main stem of Black Creek from the confluence of the forks to its mouth on the St. Johns River is 13.0 miles (20.9 km) long.

Black Creek flows into the St. Johns River 3 miles (4.8 km) north of Green Cove Springs.

It runs 14 miles (23 km) northward and then turns to the southeast flowing through Jennings State Forest to its confluence with South Fork Black Creek near Middleburg.

[4] The North Fork passes the Main Street Boat Ramp, and the approximate location of Forts Heileman and Sanderson.

Hardness, pH, and particularly, color and iron content make the water from Black Creek objectionable to most users.

[12] The Alachua Trail was an ancient Indian trail that ran from the Altamaha River in Georgia to south of the Alachua Prairie, a primary route from the Southeastern United States into north central Florida during the Second Spanish and Florida territorial periods.

The Florida Railroad, starting from Fernandina, reached Alachua County in 1860, capturing the Cotton shipments that had previously gone to Middleburg.

The project is intended to raise the water level in Lake Brooklyn and replenish the Upper Floridian aquifer.

Up to 10 million US gallons (38 Ml) a day will be captured at an intake station where Florida State Road 16 crosses the South Fork west of Penney Farms.

The District implemented a pilot project in March, 2021 to test filtering the Black Creek water through a man-made wetland.

and again crosses North Fork Black Creek 8.2 miles west of Blanding Blvd., northwest of the south entrance to Jennings State Forest.