The Black Sea Biosphere Reserve (Ukrainian: Чорноморський біосферний заповідник) is a biosphere reserve of Ukraine that is located at littoral zone of the northern Black Sea coast covering regions of the Kherson and Mykolaiv Oblasts and including Gulf of Tendra and Yahorlyk Bay.

In 1973 to the Black Sea Preserve were added the Danube floodplains, Kinski islands in Yahorlyk Bay, and the shallow portion of the Gulf of Tendra.

In 1983, there was established the Black Sea Biosphere Reserve that included the Black Sea State Preserve and the Yahorlyk State Ornithological Reserve (as its buffer zone).

The preserve was damaged in March 2022 during the Russian invasion of Ukraine, when ongoing fighting caused several fires in the area.

The reptile fauna comprises 9 species and is one of the most diverse among Ukrainian reserves.