The Blacktown Spartans Football Club are an Australian semi-professional association football club currently playing in the NSW League One, the 2nd tier of association football in New South Wales and the 3rd tier including the fully professional top tier, the A-League.

The Blacktown Spartans were formed in 2002 in the effort to field elite youth squads.

The 2010 season can be seen as successful for the Spartans, especially in the 2010 Waratah Cup as they have progressed to the semi-finals of the competition, and have been dubbed 'The Giant Killers' having knocked out the Rockdale City Suns in Round 3,[1] Sydney United in the Round of 16,[2] and Granville Rage in the Quarter Finals[3] to book a spot in the semi-finals against New South Wales Premier League side Marconi Stallions.

In 2010 the Spartans Women were promoted from the NSW Super League to the NSW Premier League after finishing on top of the Club Championship with all five teams making the semi-finals.

Following a poor 2016 NPL season, the Spartans were relegated to the second division after with just 4 wins from their 22 league games, leaving them in last place.