The larvae of Bolitophila are mycetophagous and live in decaying wood or other organic debris overgrown by fungal plant substrates.

Pupation takes place inside the fungal mycelium in soil or litter.

Adults prefer shady and humid environments and can be found in the undergrowth of mixed forests, often near watercourses.

The closest known relative to Bolitophila and only other known member of the family is the extinct genus Mangas, known from the Lower Cretaceous (Aptian) aged Gurvan-Eren Formation of Mongolia and the Khasurty locality of Buryatia, Russia.

[3] This article related to members of the fly superfamily Sciaroidea is a stub.