She has sung songs in multiple languages, including for Tamil, Telugu, Kannada, Malayalam, and Hindi movies.

Jayashri studied Bharatanatyam under Guru K. Kalyanasundaram Pillai of Shri Rajarajeshwari Bharatha Natya Kalamandir, Mumbai.

In New Delhi: Rashtrapati Bhavan, Vishnu Digambar festival, Gandharva Mahavidyalaya, Sri Shanmukhananda Sabha, India International Centre, Indira Gandhi National University, and Bhakti Utsav.

She has performed also at Saptak, Ahmedabad; The Music Academy and Sri Krishna Gana Sabha, Chennai; ITC Sangeet Research Academy, Kolkata;NCPA and Sri Shanmukhananda Sabha, Mumbai; Chowdiah Memorial Hall and Vasantha Habba, Bangalore; Kuthira Malika and Soorya Festival, Trivandrum, The Palace of Mysore and at the Matrimandir Amphitheatre at Auroville.

Jayashri's Europe tours have included venues such as: Sadler's Wells and Queen Elizabeth Hall – London; Festival of Sacred Voices and Museum Rietberg – Switzerland; Vantaa Festival and The Russian Opera House – Helsinki, Finland; Théâtre de la Ville, Festival of Nantes, The Museum of Sculpture and Arts, Musee des Asiatiques and Citi de la Musique – in France; Casa da Musica a Porto – Portugal; Sala de Camera – Spain and at the University of Cologne.

Jayashri has performed Jugalbandi concerts with Hindustani Musicians Ronu Majumdar,[11] Shubha Mudgal,[12] Vishwa Mohan Bhatt and Gaurav Mazumdar.

She has dialogued with artistes in dance like Leela Samson, Chitra Visweshwaran, Alarmel Valli, Priyadarsini Govind[13] and Shobana.

Jayashri has sung Thirukkural and an Annamacharya verse in the prestigious audio autobiography – Wings of Fire of former President Shri Abdul Kalam.

Jayashri also performed at the MTV Coke Studio (India) Season 1 with Ustad Rashid Khan and Richa Sharma.

In 2008, she was part of an Indo-Finnish venture 'Red Earth and Pouring Rain', where she sang Kuṟuntokai  – ancient poems from Sangam literature – with Avanti,conducted by John Storgards set to music by Eero Hämeenniemi.

[19] In 2012, she sang the poetry of Mirza Ghalib composed by Eero Hämeenniemi at the Vantaa festival, with the Gamba quartet led by Markku Luolajan-Mikkola, Finland.

[citation needed] Jayashri is also a playback singer whose film music includes Collaborations with M S Vishwanathan ( Tampathigal), Ilayaraja, A.R.Rahman, M M Keeravani, Johnson, Oesappachan, M Jayachandran,Ramesh Narayan, Haris Jayaraj, Shankar-Ehsan-Loy, Imman, Yuvan Shankar Raja, Govind Vasantha.

She has composed music for Kalidasa's Meghadootham, choreographed by Sheejith Nambiar and Parvathy menon, produced by Aim for Seva.

[citation needed] Jayashri featured in the first full-length Carnatic music film 'Margazhi Ragam', conceptualised by Director P Jayendra.

She has been working closely with institution like Kilikili, Sampoorna in Karnataka and Sankalp in Tamil Nadu which care for autistic children.

Some other institutions that Jayashri has worked with include: The Banyan Chennai (rehabilitation of homeless/mentally challenged women), Vasantha Memorial Trust (cancer patients),[24] Stepping Stones Orphanage Home, Malaysia, Multiple Sclerosis Society of India, Bangalore[25] and more.