[4] Unlike many of the other species of bee in the genus Bombus, B. bimaculatus is not on the decline, but instead is very stable.

[5] Additionally, B. bimaculatus can oftentimes be confused with B. impatiens and B. griseocollis, as their colorations are very similar.

bimaculatus is mainly found in eastern temperate forest regions throughout the United States and the southeastern part of Canada.

Bees do not build nests and instead rely on finding abandoned rodent dens, hollow logs, suitable man-made structures, or tussocks.

Habitats for hibernation and colonies are different so she must find a suitable location to start her nest and she must do this on her own.

[11] Eggs typically hatch after four days and spend two weeks feeding on stored provisions before pupating.

Unlike workers who stay and care for the brood, males will soon leave the nest after maturation to seek mates.

New queens are produced at about the same time as males, and will forage extensively to build reserves for their overwintering.

[6][8] B. bimaculatus is one of the earliest bumble bee species to emerge, with queens being sighted as early as February.

By the time fall arrives, newly mated queens will all be hibernating to repeat the cycle the following spring.

Though female workers are mainly responsible for brood care, males cannot fly for the first 24 hours of their life so they cannot leave the nest.

The queens hang upside down on stamens, clutch the filament with their forelegs, and scrape off pollen using their middle and hind legs.

[14] B. bimaculatus queens' probosces range from 10.53 to 12.19 mm in length, which has little overlap with other Bombus species.

[14] Research comparing B. bimaculatus to Xylocopa virginica, a carpenter bee, found that the former learned faster and had more flexible foraging patterns.

B. bimaculatus is in the same group as B. impatiens, B. griseocollis, B. affinis, B. vagans, B. sandersoni, B. perplexus and B. fraternus.

[7] As a species they have been found foraging at the following plants: Predators of bumble bees include crab spiders, Florida black bears, ambush bugs, robber flies, dragonflies, assassin bugs, and some wasp species.

[20] B. bimaculatus will defend its nests against intruders, such as Psithyrus variabilis, a cuckoo bumble bee.

Workers quickly recognized her as an intruder, halted their work and attacked her when she entered the inner part of the nest.

[23] Tracheal mites, will parasitize multiple Bombus species, but strongly prefer B. bimaculatus.

These mites can affect behavior and reduce longevity, which may cause further stress to colonies already facing difficulties.

[24] The gut bacteria of B. bimaculatus was isolated and include Snodgrassella alvi and Gilliamella apicola strains.

[28] B. bimaculatus is an important pollinator in temperate forest regions as it is still abundant, unlike many other species of honey and bumble bees.

In addition, bumble bees can continue foraging even under sub-optimal conditions such as rain or clouds.

B. bimaculatus venom contains additional proteins, including acrosin and a tryptic amidase related to clotting enzymes.