The zoo is home to nearly seventy species of wildlife including birds, mammals and reptiles, mostly representing the fauna of South America.

Besides its historical value, the main building is very representative of the era in which it was built, in terms of technology, architecture and decoration, and is registered in the "Catalogue of Buildings and Sites of Urban Planning, architectural, Historical and Artistic Heritage of the city of Asunción", and specially protected by Law 946/82 "Protection of Cultural Property".

[6] The zoo was subsequently established by the same scientists, with a very advanced approach for the time, housing the animals in a setting as close as possible to their natural habitat.

In 1936, in the aftermath of the Chaco War, a wave of xenophobic sentiment forced Fiebrig to leave Paraguay with his second wife and family.

[6] In the last 50 years, there have been several incursions, such as the riverside Empresa de Servicios Sanitarios del Paraguay (ESSAP) Viñas Cué water treatment plant, the Copaco transmission station (built at the time of dictator Stroessner), the Asunción Golf Club, laid out by Fiebrig, and several other divisions due to illegal occupations.

Among its collections are:[citation needed] On 4 May 2006 the gardens launched the exhibition Ethnobotany 2006 "Our plants, our people" ("Nuestras Plantas, Nuestra Gente"), under the Paraguayan Ethnobotany Project (EPY) (which lasted for about ten years), with the support of the Conservatory and Botanical Garden of the City of Geneva and under the auspices of the organization Tesãi Reka Paraguay (TRP).