is the federal district court whose territorial jurisdiction spans five counties in New York State: the four Long Island counties of Nassau, Suffolk, Kings (Brooklyn), and Queens, as well as Richmond (Staten Island), the latter three being among New York City's five boroughs.

The acting United States Attorney for the Eastern District of New York since January 2025 is Carolyn Pokorny.

The main location is the Theodore Roosevelt United States Courthouse at 225 Cadman Plaza East in the civic center of Brooklyn.

The courthouse was designed in 1995 but did not open until 2006 following redesign requirements in the wake of the Oklahoma City bombing and the September 11 attacks.

It replaced the six story Emanuel Celler Federal Building (built in 1962 and located next door and connected via glass atrium).

The Divisional office is in the Alfonse M. D'Amato United States Courthouse in Central Islip, New York.