Founded in 2008, the team played only one season (2009) in the National Premier Soccer League (NPSL), a national premier league at the fourth tier of the American Soccer Pyramid, in the Eastern Keystone Division.

They sold their rights as a club in the league to another group in Western New York, who then formed FC Buffalo.

The team played all its 2009 home games at the stadium on the campus of Nichols School.

After just one season in the NPSL, in which the team finished second in the Keystone Conference, Buffalo City FC sold their franchise rights to FC Buffalo, who took their place in the NPSL.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.