The Buffalo Presbyterian Church and Cemetery in Greensboro, Guilford County, North Carolina, United States, is a historic Presbyterian church complex and cemetery located at 800 and 803 Sixteenth Street in Greensboro.

The Federal-style church sanctuary was built in 1827, and updated and expanded in 1919–1920 in the Colonial Revival style by architect Harry Barton (1876-1937).

Flanking the sanctuary are two-story Colonial Revival-style educational buildings linked to it by one-story arcades.

The rectangular two-story Colonial Revival-style manse and garage were added to the complex in 1924.

The church cemetery has burials dating back to 1775.