[2] The paired glands are found adjacent to the urethra just below the prostate, seen best by screening (medicine) MRI as a tool in preventative healthcare in males.

Screening MRI may be performed when there is a positive prostate-specific antigen on basic laboratory tests.

[6] Bulbourethral glands are located posterior and lateral to the membranous portion of the urethra at the base of the penis, between the two layers of the fascia of the urogenital diaphragm, in the deep perineal pouch.

[8][unreliable source] The bulbourethral gland contributes up to 4 ml of fluid during sexual arousal.

However, the sperm source is a residual or pre-ejaculatory leak from the testicles into the vasa deferentia,[9] rather than from the bulbourethral gland itself.